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GrainGenes Marker Report: Xgwm149-4B

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Locus	Xgwm149-4B
Type	Microsatellite
Chromosome	4B
Chromosome Arm	4BL
Map	Chinese_Spring_Deletion_SSR_4B 0.93 [ Show all 8 ]
	[ Show Nearby Loci ]
Breakpoint Interval	4BL1-0.86-1.00
Map Data	Wheat, Chinese Spring x SQ1 [ Show all 8 ]
Probe	WMS149
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Somers DJ et al. (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat Genome 46:555-564. Sukhwinder S et al. (2003) Mapping of a resistance gene effective against Karnal bunt pathogen of wheat Theoretical and Applied Genetics 106:287-292. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-4B, the interval most likely to contain locus is between Xcdo1401 and Xcdo1312
Possible Orthologs	Xgwm149 Xgwm149-4B.1 gwm149 Xgwm149-4B.1-2 gwm149-4B
Probe for Locus	WMS149
Locus	Xgwm149-4B [ Show all 6 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)23imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CATTGTTTTCTGCCTCTAGCC 3' 5' CTAGCATCGAACCTGAACAAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS149
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring