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GrainGenes Marker Report: Xgwm153

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Locus	Xgwm153
Type	Microsatellite
Chromosome	1B
Map	Svevo/Ciccio-1B 164.5 Ta-NxW-1B 122.5 Wheat-Composite2004-1B 77
	[ Show Nearby Loci ]
Breakpoint Interval	1BL2-0.69-0.85
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMS153
Possible Orthologs	Xgwm153-1B gwm153 gwm153-1B
Probe for Locus	WMS153
Locus	Xgwm153-1B Xgwm153 gwm153 gwm153-1B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)18 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GATCTCGTCACCCGGAATTC 3' 5' TGGTAGAGAAGGACGGAGAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS153
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring