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GrainGenes Marker Report: Xgwm153-1B

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Locus	Xgwm153-1B
Type	Microsatellite
Chromosome	1B
Chromosome Arm	1BL
Map	Chinese_Spring_Deletion_SSR_1B 0.845 [ Show all 9 ]
	[ Show Nearby Loci ]
Breakpoint Interval	1BL2-0.69-1.00*
Map Data	Durum wheat, Svevo x Ciccio [ Show all 10 ]
Species	Triticum aestivum
Probe	WMS153
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-1B, the intervals most likely to contain locus are Xbcd442 - Xbcd304 or Xbcd304 - XksuG34
Possible Orthologs	Xgwm153 gwm153 gwm153-1B
Probe for Locus	WMS153
Locus	Xgwm153-1B Xgwm153 gwm153 gwm153-1B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)18 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GATCTCGTCACCCGGAATTC 3' 5' TGGTAGAGAAGGACGGAGAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS153
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring