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GrainGenes Marker Report: Xgwm154b

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Locus	Xgwm154b
Type	Microsatellite
Chromosome	5A
Map	Wheat-Composite2004-5A 40
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS154
Possible Orthologs	Xgwm154 [ Show all 7 ]
Probe for Locus	WMS154
Locus	Xgwm154 [ Show all 8 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)37imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCACAGAGAGAGAGGGAGGG 3' 5' ATGTGTACATGTTGCCTGCA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS154
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring