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GrainGenes Marker Report: Xgwm157

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Locus	Xgwm157
Type	Microsatellite
Chromosome	2D
Map	Wheat-2008-Corrigin-CS(T2D)-2D 88 Wheat-Composite2004-2D 59
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Map Data	Wheat 2008, Corrigin x CS(T2D), C gene Wheat, Composite, 2004
Probe	WMS157
Possible Orthologs	Xgwm157-2D gwm157 gwm157-2D
Probe for Locus	WMS157
Locus	Xgwm157-2D Xgwm157 Acph-D2 gwm157 gwm157-2D
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)14 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GTCGTCGCGGTAAGCTTG 3' 5' GAGTGAACACACGAGGCTTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS157
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring