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GrainGenes Marker Report: Xgwm16-2B

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Locus	Xgwm16-2B
Type	Microsatellite
Chromosome	2B
Map	Chinese_Spring_Deletion_SSR_2B 0.18 [ Show all 6 ]
	[ Show Nearby Loci ]
Breakpoint Interval	C-2BL2-0.36
Map Data	Wheat, Consensus SSR, 2004 [ Show all 6 ]
Probe	WMS16
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157. Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Possible Orthologs	Xgwm16 [ Show all 8 ]
Probe for Locus	WMS16
Locus	Xgwm16-2B [ Show all 9 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (C)12ACAAA(CA)14(GA)18 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GCTTGGACTAGCTAGAGTATCATAC 3' 5' CAATCTTCAATTCTGTCGCACGG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS16
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring