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GrainGenes Marker Report: Xgwm162

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Locus	Xgwm162
Type	Microsatellite
Map	Svevo/Ciccio-3A 87.9 Ta-NxW-3A 196.2
	[ Show Nearby Loci ]
QTL with Locus Marker	QGwt.crc-4A
Breakpoint Interval	C-3AL3-0.42
Map Data	Durum wheat, Svevo x Ciccio Wheat, Nanda2419 x Wangshuibai
Probe	WMS162
Possible Orthologs	Xgwm162-3A [ Show all 6 ]
Probe for Locus	WMS162
Locus	Xgwm162-3A [ Show all 7 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)14AA(CA)4 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AGTGGATCGACAAGGCTCTG 3' 5' AGAAGAAGCAAAGCCTTCCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS162
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring