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GrainGenes Marker Report: Xgwm164

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Locus	Xgwm164
Type	Microsatellite
Chromosome	1A
Map	Wheat, Avalon x Cadenza 1A 59.2 Wheat-Composite2004-1A 57
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Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004
Probe	WMS164
Possible Orthologs	Xgwm164-1A gwm164 gwm164-1A
Probe for Locus	WMS164
Locus	Xgwm164-1A Xgwm164 gwm164 gwm164-1A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)16 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ACATTTCTCCCCCATCGTC 3' 5' TTGTAAACAAATCGCATGCG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS164
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring