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GrainGenes Marker Report: Xgwm169

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Locus	Xgwm169
Type	Microsatellite
Chromosome	6A
Map	Ta-NxW-6A 110.6 Wheat, Avalon x Cadenza 6A 119.5 Wheat-Composite2004-6A 127
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Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMS169
Possible Orthologs	Xgwm169-6A gwm169
Probe for Locus	WMS169
Locus	Xgwm169-6A Xgwm169 gwm169
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)23 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ACCACTGCAGAGAACACATACG 3' 5' GTGCTCTGCTCTAAGTGTGGG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS169
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring