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GrainGenes Marker Report: Xgwm174

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Locus	Xgwm174
Type	Microsatellite
Chromosome	5D
Map	Wheat, Avalon x Cadenza 5D 68.9 Wheat, UC1110 x PI610750 5D 91.8 Wheat-Composite2004-5D 52
	[ Show Nearby Loci ]
Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMS174
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm174-5D gwm174 gwm174-5D gwm174-6B
Probe for Locus	WMS174
Locus	Xgwm174-5D Xgwm174 gwm174 gwm174-5D gwm174-6B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GGGTTCCTATCTGGTAAATCCC 3' 5' GACACACATGTTCCTGCCAC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS174
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring