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GrainGenes Marker Report: Xgwm174-5D

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Locus	Xgwm174-5D
Type	Microsatellite
Chromosome	5D
Chromosome Arm	5DL
Map	Chinese_Spring_Deletion_SSR_5D 0.67 [ Show all 10 ]
	[ Show Nearby Loci ]
Breakpoint Interval	5DL1-0.60-0.74
Map Data	Wheat, Arina x Forno [ Show all 10 ]
Species	Triticum aestivum
Probe	WMS174
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Possible Orthologs	Xgwm174 gwm174 gwm174-5D gwm174-6B
Probe for Locus	WMS174
Locus	Xgwm174-5D Xgwm174 gwm174 gwm174-5D gwm174-6B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GGGTTCCTATCTGGTAAATCCC 3' 5' GACACACATGTTCCTGCCAC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS174
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring