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GrainGenes Marker Report: Xgwm181

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Locus	Xgwm181
Type	Microsatellite
Chromosome	3B
Chromosome Arm	3BL
Map	Tt-LxG-3B 217.3 Wheat-Composite2004-3B 99 Wheat_YrH922_2019_exp 22.8
	[ Show Nearby Loci ]
QTL with Locus Marker	QPhs.hwwgr-3BL
Map Data	T. turgidum, Langdon x G18-16 Wheat, Composite, 2004 Wheat_YrH922_2019
Probe	WMS181
Possible Orthologs	gwm181 Xgwm181-3B gwm181-3B
Probe for Locus	WMS181
Locus	Xgwm181 Xgwm181-3B gwm181 gwm181-3B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)28 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCATTGGTAATGAGGAGAGA 3' 5' GAACCATTCATGTGCATGTC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS181
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring