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GrainGenes Marker Report: Xgwm181-3B

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Locus	Xgwm181-3B
Type	Microsatellite
Chromosome	3B
Chromosome Arm	3BL
Map	Chinese_Spring_Deletion_SSR_3B 0.815 [ Show all 10 ]
	[ Show Nearby Loci ]
Breakpoint Interval	3BL7-0.63-1.00
Map Data	Durum wheat, Kofa x UC1113 [ Show all 10 ]
Species	Triticum aestivum
Probe	WMS181
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-3B, the interval most likely to contain locus is between Xtam63 and Xmwg11
Possible Orthologs	gwm181 Xgwm181 gwm181-3B
Probe for Locus	WMS181
Locus	Xgwm181 Xgwm181-3B gwm181 gwm181-3B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)28 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCATTGGTAATGAGGAGAGA 3' 5' GAACCATTCATGTGCATGTC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS181
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring