Query (optional)   in Class  

GrainGenes Marker Report: Xgwm194

[Submit comment/correction]

Locus
Xgwm194
Type
Microsatellite
Chromosome
4D
Map
Wheat, Avalon x Cadenza 4D56.3
Wheat, UC1110 x PI610750 4D122.8
Wheat-Composite2004-4D100
[ Show Nearby Loci ]
Map Data
Wheat, Avalon x Cadenza
Wheat, Composite, 2004
Wheat, UC1110 x PI610750
Probe
WMS194
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
Xgwm194-4D
gwm194



Probe for Locus
WMS194
Locus
Xgwm194-4D
Xgwm194
gwm194
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)32imp
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GATCTGCTCTACTCTCCTCC 3'
5' CGACGCAGAACTTAAACAAG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS194
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring