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GrainGenes Marker Report: Xgwm212

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Locus	Xgwm212
Type	Microsatellite
Chromosome	5D
Map	Ta-NxW-5D 149.1 Wheat-Composite2004-5D 72
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMS212
Possible Orthologs	Xgwm212-5D Xgwm212-5R gwm212 gwm212-5D
Probe for Locus	WMS212
Locus	Xgwm212-5D Xgwm212 Xgwm212-5R gwm212 gwm212-5D
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AAGCAACATTTGCTGCAATG 3' 5' TGCAGTTAACTTGTTGAAAGGA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS212
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring