GrainGenes Marker Report: Xgwm212-5D
Locus
Xgwm212-5D
Type
Microsatellite
Chromosome
5D
Map
Chinese_Spring_Deletion_SSR_5D 0.88
[ Show all 7 ]
[ Show Nearby Loci ]
Breakpoint Interval
5DL5-0.76-1.00
Map Data
Wheat, Chinese Spring x SQ1
[ Show all 7 ]
Probe
WMS212
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-5D, the interval most likely to contain locus is between Xbcd450 and Xmwg922
Possible Orthologs
Xgwm212 Xgwm212-5R gwm212 gwm212-5D
Probe for Locus
WMS212
Locus
Xgwm212-5D Xgwm212 Xgwm212-5R gwm212 gwm212-5D
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' AAGCAACATTTGCTGCAATG 3'
5' TGCAGTTAACTTGTTGAAAGGA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS212
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm212-5D
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