GrainGenes Marker Report: Xgwm212-5R
Locus
Xgwm212-5R
Type
Microsatellite
Chromosome
5R
Map
Secale-PxP-2004-5R 137.9
[ Show Nearby Loci ]
Map Data
Rye, P87 x P105, 2004
Species
Secale cereale
Probe
WMS212
Reference
Khlestkina EK et al. (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags Theoretical and Applied Genetics 109:725-732.
Possible Orthologs
Xgwm212 Xgwm212-5D gwm212 gwm212-5D
Probe for Locus
WMS212
Locus
Xgwm212-5D Xgwm212 Xgwm212-5R gwm212 gwm212-5D
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' AAGCAACATTTGCTGCAATG 3'
5' TGCAGTTAACTTGTTGAAAGGA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS212
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm212-5R
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