GrainGenes Marker Report: Xgwm219.2
Locus
Xgwm219.2
Type
Microsatellite
Map
Ta-NxW-6B 128.7
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
WMS219
Possible Orthologs
Xgwm219 Xgwm219-6B Xgwm219.1 gwm219 gwm219-6B
Probe for Locus
WMS219
Locus
Xgwm219-6B
[ Show all 6 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)35imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' GATGAGCGACACCTAGCCTC 3'
5' GGGGTCCGAGTCCACAAC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS219
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm219.2
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