GrainGenes Marker Report: Xgwm232-5D
Locus
Xgwm232-5D
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_5D 0.3
[ Show Nearby Loci ]
Breakpoint Interval
C-5DL1-0.60
Map Data
Wheat, Physical, SSR
Probe
WMS232
Possible Orthologs
Xgwm232 Xgwm232-1D gwm232 gwm232-1D
Probe for Locus
WMS232
Locus
Xgwm232-1D Xgwm232 Xgwm232-5D gwm232 gwm232-1D
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)19 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' ATCTCAACGGCAAGCCG 3'
5' CTGATGCAAGCAATCCACC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS232
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm232-5D
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