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GrainGenes Marker Report: Xgwm233

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Locus	Xgwm233
Type	Microsatellite
Chromosome	7A 7B
Map	Wheat-Composite2004-7A 3 Wheat-Composite2004-7B 17
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Map Data	Wheat, Composite, 2004
Probe	WMS233
Possible Orthologs	Xgwm233-7A gwm233 gwm233-7A
Probe for Locus	WMS233
Locus	Xgwm233-7A Xgwm233 gwm233 gwm233-7A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)24 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCAAAACATAAATGTTCATTGGA 3' 5' TCAACCGTGTGTAATTTTGTCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS233
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring