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GrainGenes Marker Report: Xgwm234-5B

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Locus	Xgwm234-5B
Type	Microsatellite
Chromosome	5B
Chromosome Arm	5BS
Map	Chinese_Spring_Deletion_SSR_5B -0.76 [ Show all 12 ]
	[ Show Nearby Loci ]
Breakpoint Interval	5BS5-0.71-0.81
Map Data	Durum wheat, Kofa x UC1113 [ Show all 13 ]
Species	Triticum aestivum
Probe	WMS234
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-5B, the intervals most likely to contain locus are Xbcd873 - Xgwm443 or distal to Xbcd873
Possible Orthologs	Xgwm234 [ Show all 9 ]
Probe for Locus	WMS234
Locus	Xgwm234-5B [ Show all 10 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)16(CA)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GAGTCCTGATGTGAAGCTGTTG 3' 5' CTCATTGGGGTGTGTACGTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS234
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring