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GrainGenes Marker Report: Xgwm247.1-1B

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Locus	Xgwm247.1-1B
Type	Microsatellite
Map	Louise/Penawawa-1B 1.6
	[ Show Nearby Loci ]
Map Data	Wheat, Louise x Penawawa
Probe	WMS247
Possible Orthologs	Xgwm247 [ Show all 7 ]
Probe for Locus	WMS247
Locus	Xgwm247 [ Show all 8 ]
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)24 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. In 369 Avena sterilis accessions there were 4 alleles of size 39-60 bp, PIC = 0.04.
Type	Genomic PCR SSR
PCR primers	5' GCAATCTTTTTTCTGACCACG 3' 5' ATGTGCATGTCGGACGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS247
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring