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GrainGenes Marker Report: Xgwm249-2D

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Locus	Xgwm249-2D
Type	Microsatellite
Chromosome	2D
Map	Ta-Courtot/CS-GPW-2D 110.9 Ta-SSR-2004-2D 63.6 Ta-Synthetic/Opata-BARC-2D 49.8 Ta-Synthetic/Opata-GPW-2D 132.2 Ta-Synthetic/Opata-SSR-2D 92.7
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe	WMS249
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-2D, the intervals most likely to contain locus are Xgwm515 - Xbcd260 or Xbcd260 - Xbcd111
Possible Orthologs	Xgwm249 [ Show all 10 ]
Probe for Locus	WMS249
Locus	Xgwm249-2D [ Show all 11 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)11(GGA)8 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CAAATGGATCGAGAAAGGGA 3' 5' CTGCCATTTTTCTGGATCTACC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS249
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring