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GrainGenes Marker Report: Xgwm251

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Locus	Xgwm251
Type	Microsatellite
Chromosome	4B 4D
Chromosome Arm	4BL
Map	Svevo/Ciccio-4B 163.5 Tt-LxG-4B 34 Wheat, UC1110 x PI610750 4B 44 Wheat-Composite2004-4B 45 Wheat-Composite2004-4D 136
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Breakpoint Interval	4BL5-0.86-1.00
Map Data	Durum wheat, Svevo x Ciccio T. turgidum, Langdon x G18-16 Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMS251
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm251-4B gwm251 gwm251-4B
Probe for Locus	WMS251
Locus	Xgwm251 Xgwm251-4B Yr62 gwm251 gwm251-4B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)28 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CAACTGGTTGCTACACAAGCA 3' 5' GGGATGTCTGTTCCATCTTAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS251
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring