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GrainGenes Marker Report: Xgwm260

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Locus	Xgwm260
Type	Microsatellite
Chromosome	7A
Map	Pgene-durum-x-polonicum_7A 9.6 Wheat-Composite2004-7A 110
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Map Data	Wheat, Composite, 2004 Wheat, P Gene, 2002
Probe	WMS260
Possible Orthologs	Xgwm260-7A gwm260
Probe for Locus	WMS260
Locus	Xgwm260-7A Xgwm260 P1 gwm260
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GCCCCCTTGCACAAATC 3' 5' CGCAGCTACAGGAGGCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS260
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring