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GrainGenes Marker Report: Xgwm261-2D

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Locus	Xgwm261-2D
Type	Microsatellite
Chromosome	2D
Chromosome Arm	2DS
Map	Chinese_Spring_Deletion_SSR_2D -0.165 [ Show all 9 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	QFhs.pur-2D
Breakpoint Interval	C-2DS1-0.33
Map Data	Wheat, Arina x Forno [ Show all 10 ]
2 Point Data	Rht8/Xgwm261 Distance: 0.6 cM
Species	Triticum aestivum
Probe	WMS261
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Somers DJ et al. (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat Genome 46:555-564. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Possible Orthologs	Xgwm261 gwm261
Probe for Locus	WMS261
Locus	Xgwm261-2D Xgwm261 Rht8 gwm261
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CTCCCTGTACGCCTAAGGC 3' 5' CTCGCGCTACTAGCCATTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS261
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring