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GrainGenes Marker Report: Xgwm269-5D

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Locus	Xgwm269-5D
Type	Microsatellite
Chromosome	5D
Chromosome Arm	5DL
Map	Chinese_Spring_Deletion_SSR_5D 0.88 [ Show all 7 ]
	[ Show Nearby Loci ]
Breakpoint Interval	5DL5-0.76-1.00
Map Data	Wheat, Arina x Forno [ Show all 7 ]
Species	Triticum aestivum
Probe	WMS269
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-5D, the intervals most likely to contain locus are Xbcd1421 - Xbcd197 or distal to Xbcd197
Possible Orthologs	Xgwm269 [ Show all 7 ]
Probe for Locus	WMS269
Locus	Xgwm269.2-4A [ Show all 8 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)29 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TGCATATAAACAGTCACACACCC 3' 5' TTTGAGCTCCAAAGTGAGTTAGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS269
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring