GrainGenes Marker Report: Xgwm271-2B
Locus
Xgwm271-2B
Type
Microsatellite
Chromosome
2B
Map
Ta-SSR-2004-2B 64.5 USG3209/Jaypee-2B 74.1
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004 Wheat, USG3209 x Jaypee
Probe
WMS271
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xgwm271
[ Show all 14 ]
Probe for Locus
WMS271
Locus
Xgwm271-5B
[ Show all 16 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)4imp(GA)10 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' CAAGATCGTGGAGCCAGC 3'
5' AGCTGCTAGCTTTTGGGACA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS271
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm271-2B
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