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GrainGenes Marker Report: Xgwm274-7B

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Locus	Xgwm274-7B
Type	Microsatellite
Chromosome	7B
Map	Ta-SSR-2004-7B 76.7 Ta-Synthetic/Opata-GPW-7B 61.4 Ta-Synthetic/Opata-SSR-7B 92.55
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe	WMS274
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-7B, the interval most likely to contain locus is between Xwg686 and XksuD2
Possible Orthologs	gwm274 [ Show all 14 ]
Probe for Locus	WMS274
Locus	Xgwm274-1B [ Show all 15 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)27 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AACTTGCAAAACTGTTCTGA 3' 5' TATTTGAAGCGGTTTGATTT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS274
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring