GrainGenes Marker Report: Xgwm274.1
Locus
Xgwm274.1
Type
Microsatellite
Chromosome
1B
Map
Ta-NxW-3B 109.9 Wheat-Composite2004-1B 77
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe
WMS274
Possible Orthologs
gwm274
[ Show all 14 ]
Probe for Locus
WMS274
Locus
Xgwm274-1B
[ Show all 15 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)27 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' AACTTGCAAAACTGTTCTGA 3'
5' TATTTGAAGCGGTTTGATTT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS274
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm274.1
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