GrainGenes Marker Report: Xgwm275-2A.2
Locus
Xgwm275-2A.2
Type
Microsatellite
Map
USG3209/Jaypee-2A 101
[ Show Nearby Loci ]
Map Data
Wheat, USG3209 x Jaypee
Probe
WMS275
Possible Orthologs
Xgwm275
[ Show all 12 ]
Probe for Locus
WMS275
Locus
Xgwm275
[ Show all 13 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' AATTTTCTTCCTCACTTATTCT 3'
5' AACAAAAAATTAGGGCC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS275
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm275-2A.2
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