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GrainGenes Marker Report: Xgwm285b

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Locus	Xgwm285b
Type	Microsatellite
Chromosome	3B
Map	Wheat-Composite2004-3B 53
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS285
Possible Orthologs	Xgwm285-3B gwm285 Xgwm285 gwm285-3B
Probe for Locus	WMS285
Locus	Xgwm285-3B [ Show all 6 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)27 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ATGACCCTTCTGCCAAACAC 3' 5' ATCGACCGGGATCTAGCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS285
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring