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GrainGenes Marker Report: Xgwm297

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Locus	Xgwm297
Type	Microsatellite
Chromosome	7B
Map	Ta-NxW-7B 37.5 Wheat, UC1110 x PI610750 7B 94.7 Wheat-2002-ColeoptileColor-7B 48.4 Wheat-Composite2004-7B 44 Wheat_Yr79_2018-7BL 53
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Map Data	Wheat, 2002, coleoptile color Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750 Wheat_Yr79_2018
Probe	WMS297
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm297-7B gwm297 gwm297-7B
Probe for Locus	WMS297
Locus	Xgwm297-7B Xgwm297 gwm297 gwm297-7B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)12(GA)18 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ATCGTCACGTATTTTGCAATG 3' 5' TGCGTAAGTCTAGCATTTTCTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS297
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring