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GrainGenes Marker Report: Xgwm299-3B

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Locus
Xgwm299-3B
Type
Microsatellite
Chromosome
3B
Chromosome Arm
3BL
Map
Conan/Reeder-3B225.633
[ Show all 10 ]
[ Show Nearby Loci ]
Map Data
Durum wheat, Svevo x Ciccio
[ Show all 11 ]
Probe
WMS299
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
ReferenceSomers DJ et al. (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat Genome 46:555-564.
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source
Roeder, Marion S.98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-3B, the interval most likely to contain locus is between Xbg131 and Xfba235
Possible Orthologs
gwm299
Xgwm299
Xgwm299-2B
Xgmw299
gwm299-3B



Probe for Locus
WMS299
Locus
Xgwm299-3B
[ Show all 6 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)31(TAG)4
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' ACTACTTAGGCCTCCCGCC 3'
5' TGACCCACTTGCAATTCATC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS299
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring