GrainGenes Marker Report: Xgwm3-3D
Locus
Xgwm3-3D
Type
Microsatellite
Chromosome
3D
Chromosome Arm
3DL
Map
Chinese_Spring_Deletion_SSR_3D 0.135
[ Show all 9 ]
[ Show Nearby Loci ]
Breakpoint Interval
C-3DL2-0.27
Map Data
Wheat, Conan x Reeder
[ Show all 9 ]
Probe
WMS3
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-3D, the interval most likely to contain locus is between Xfbb269 and XksuG59
Possible Orthologs
Xgwm3 Xgwm3-2D Xgwm3b gwm3
Probe for Locus
WMS3
Locus
Xgwm3-3D
[ Show all 7 ]
Reference
Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038.
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks
Insert contains repeat (CA)18 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeat type (CA)x18. Product size 84 bp in Cheyenne. In 369 Avena sterilis accessions there were 3 alleles of size 44-50 bp, PIC = negative.
Type
Genomic PCR SSR
PCR primers
5' GCAGCGGCACTGGTACATTT 3'
5' AATATCGCATCACTATCCCA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS3
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm3-3D
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