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GrainGenes Marker Report: Xgwm304

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Locus	Xgwm304
Type	Microsatellite
Chromosome	5A
Map	Svevo/Ciccio-2A 107.9 Ta-NxW-5A 70.1 Wheat, Finch x Eltan 5A 48.4 Wheat, UC1110 x PI610750 5A 41.5 Wheat-Composite2004-5A 24
	[ Show Nearby Loci ]
QTL with Locus Marker	QFelt.wpg-5A.2
Breakpoint Interval	C-2AL1-0.85
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004 Wheat, Finch x Eltan Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMS304
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm304-2A [ Show all 7 ]
Probe for Locus	WMS304
Locus	Xgwm304.2-2A [ Show all 8 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AGGAAACAGAAATATCGCGG 3' 5' AGGACTGTGGGGAATGAATG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS304
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring