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GrainGenes Marker Report: Xgwm312-2A

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Locus	Xgwm312-2A
Type	Microsatellite
Chromosome	2A
Chromosome Arm	2AL
Map	Conan/Reeder-2A 374.958 [ Show all 9 ]
	[ Show Nearby Loci ]
Map Data	Wheat, Arina x Forno [ Show all 9 ]
Species	Triticum aestivum
Probe	WMS312
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-2A, the intervals most likely to contain locus are Xbcd543 - XksuE16 or XksuE16 - Xpsr934
Possible Orthologs	Xgwm312 gwm312 gwm312-2A
Probe for Locus	WMS312
Locus	Xgwm312-2A [ Show all 7 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)37 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ATCGCATGATGCACGTAGAG 3' 5' ACATGCATGCCTACCTAATGG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS312
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring