GrainGenes Marker Report: Xgwm320-2R
Locus
Xgwm320-2R
Type
Microsatellite
Chromosome
2R
Map
Secale-PxP-2004-2R 196.8
[ Show Nearby Loci ]
Map Data
Rye, P87 x P105, 2004
Species
Secale cereale
Probe
WMS320
Reference
Khlestkina EK et al. (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags Theoretical and Applied Genetics 109:725-732.
Possible Orthologs
Xgwm320 Xgwm320-2D gwm320
Probe for Locus
WMS320
Locus
Xgwm320-2D Xgwm320 Xgwm320-2R gwm320
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)9(GA)15 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' CGAGATACTATGGAAGGTGAGG 3'
5' ATCTTTGCAAGGATTGCCC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS320
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm320-2R
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