GrainGenes Marker Report: Xgwm33-1B
Locus
Xgwm33-1B
Type
Microsatellite
Chromosome
1B
Chromosome Arm
1BL
Map
Chinese_Spring_Deletion_SSR_1B -1.325 Ta-SSR-2004-1B 28 Ta-Synthetic/Opata-BARC-1B 10.7 Ta-Synthetic/Opata-GPW-1B 18.9 Ta-Synthetic/Opata-SSR-1B 7.3
[ Show Nearby Loci ]
Breakpoint Interval
1BS.sat18-0.50-1.00
Map Data
Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe
WMS33
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-1B, the interval most likely to contain locus is between XksuD14 and Xmwg938
Possible Orthologs
Xgwm33
[ Show all 13 ]
Probe for Locus
WMS33
Locus
Xgwm33-1A
[ Show all 14 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks
Insert contains repeat (GA)19 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeat type (GA)x19. Product size 123 bp in Chinese Spring.
Type
PCR SSR
PCR primers
5' GGAGTCACACTTGTTTGTGCA 3'
5' CACTGCACACCTAACTACCTGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS33
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm33-1B
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