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GrainGenes Marker Report: Xgwm33.2

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Locus	Xgwm33.2
Type	Microsatellite
Chromosome	1B
Map	Wheat-Composite2004-1B 19
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS33
Possible Orthologs	Xgwm33 [ Show all 13 ]
Probe for Locus	WMS33
Locus	Xgwm33-1A [ Show all 14 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks	Insert contains repeat (GA)19 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeat type (GA)x19. Product size 123 bp in Chinese Spring.
Type	PCR SSR
PCR primers	5' GGAGTCACACTTGTTTGTGCA 3' 5' CACTGCACACCTAACTACCTGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS33
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring