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GrainGenes Marker Report: Xgwm332b

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Locus
Xgwm332b
Type
Microsatellite
Chromosome
7A
Map
Wheat-Composite2004-7A202
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMS332
Possible Orthologs
Xgwm332
[ Show all 7 ]



Probe for Locus
WMS332
Locus
Xgwm332-7A
[ Show all 8 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)36
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' AGCCAGCAAGTCACCAAAAC 3'
5' AGTGCTGGAAAGAGTAGTGAAGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS332
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring