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GrainGenes Marker Report: Xgwm333

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Locus	Xgwm333
Type	Microsatellite
Chromosome	7B
Map	Svevo/Ciccio-7B 82.1 Ta-NxW-7B 39.8 Wheat-Composite2004-7B 46 Wheat_Fhb7_2020 0 Wheat_Yr79_2018-7BL 54.6
	[ Show Nearby Loci ]
Breakpoint Interval	C-7BL7-0.63
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat_Yr79_2018
Probe	WMS333
Possible Orthologs	Xgwm333-7B gwm333 gwm333-7B
Probe for Locus	WMS333
Locus	Xgwm333-7B Xgwm333 gwm333 gwm333-7B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)19 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GCCCGGTCATGTAAAACG 3' 5' TTTCAGTTTGCGTTAAGCTTTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS333
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring