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GrainGenes Marker Report: Xgwm334

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Locus	Xgwm334
Type	Microsatellite
Chromosome	6A
Map	Svevo/Ciccio-6A 16.1 Wheat, Avalon x Cadenza 6A 15.3 Wheat-Composite2004-6A 28
	[ Show Nearby Loci ]
Breakpoint Interval	6AS1-0.35-1.00
Map Data	Durum wheat, Svevo x Ciccio Wheat, Avalon x Cadenza Wheat, Composite, 2004
Probe	WMS334
Possible Orthologs	Xgwm334-6A gwm334 gwm334-6A
Probe for Locus	WMS334
Locus	Xgwm334-6A Xgwm334 gwm334 gwm334-6A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)19 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AATTTCAAAAAGGAGAGAGA 3' 5' AACATGTGTTTTTAGCTATC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS334
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring