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GrainGenes Marker Report: Xgwm334-6A

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Locus	Xgwm334-6A
Type	Microsatellite
Chromosome	6A
Chromosome Arm	6AS
Map	Chinese_Spring_Deletion_SSR_6A -0.675 [ Show all 6 ]
	[ Show Nearby Loci ]
Breakpoint Interval	6AS1-0.35-1.00*
Map Data	Durum wheat, Svevo x Ciccio [ Show all 7 ]
Probe	WMS334
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-6A, the interval most likely to contain locus is between Xbcd1821 and XksuG48
Possible Orthologs	Xgwm334 gwm334 gwm334-6A
Probe for Locus	WMS334
Locus	Xgwm334-6A Xgwm334 gwm334 gwm334-6A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)19 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AATTTCAAAAAGGAGAGAGA 3' 5' AACATGTGTTTTTAGCTATC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS334
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring