GrainGenes Marker Report: Xgwm335-5R
Locus
Xgwm335-5R
Type
Microsatellite
Chromosome
5R
Map
Secale-PxP-2004-5R 72.4
[ Show Nearby Loci ]
Map Data
Rye, P87 x P105, 2004
Species
Secale cereale
Probe
WMS335
Reference
Khlestkina EK et al. (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags Theoretical and Applied Genetics 109:725-732.
Possible Orthologs
Xgwm335 Xgwm335-5B gwm335 gwm335-5B
Probe for Locus
WMS335
Locus
Xgwm335-5B Xgwm335 Xgwm335-5R gwm335 gwm335-5B
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)14(GCGT)3 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' CGTACTCCACTCCACACGG 3'
5' CGGTCCAAGTGCTACCTTTC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS335
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm335-5R
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