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GrainGenes Marker Report: Xgwm339

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Locus
Xgwm339
Type
Microsatellite
Chromosome
2A
Chromosome Arm
2AS
Map
Svevo/Ciccio-2A77.6
Ta-NxW-2A151.6
Tt-LxG-2A51.4
Wheat-Composite2004-2A56
[ Show Nearby Loci ]
Breakpoint Interval
C-2AS5-0.78
Map Data
Durum wheat, Svevo x Ciccio
T. boeoticum x monococcum
T. turgidum, Langdon x G18-16
Wheat, Composite, 2004
Wheat, Nanda2419 x Wangshuibai
Probe
WMS339
Possible Orthologs
Xgwm339-2A
gwm339
gwm339-2A



Probe for Locus
WMS339
Locus
Xgwm339-2A
Xgwm339
gwm339
gwm339-2A
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)22
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' AATTTTCTTCCTCACTTATT 3'
5' AAACGAACAACCACTCAATC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS339
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring