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GrainGenes Marker Report: Xgwm340

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Locus	Xgwm340
Type	Microsatellite
Chromosome	3B
Map	Svevo/Ciccio-3B 196.2 Wheat, UC1110 x PI610750 3B 131.7 Wheat-Composite2004-3B 98
	[ Show Nearby Loci ]
Breakpoint Interval	3BL7-0.63-1.00
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMS340
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm340-3B Xgwm340-6R gwm340 gwm340-3B
Probe for Locus	WMS340
Locus	Xgwm340-3B Xgwm340 Xgwm340-6R gwm340 gwm340-3B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)26 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GCAATCTTTTTTCTGACCACG 3' 5' ACGAGGCAAGAACACACATG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS340
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring