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GrainGenes Marker Report: Xgwm350

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Locus	Xgwm350
Type	Microsatellite
Map	Svevo/Ciccio-4A 120.5 Wheat, Avalon x Cadenza 4A2 0
	[ Show Nearby Loci ]
Breakpoint Interval	4AL4-0.80-1.00
Map Data	Durum wheat, Svevo x Ciccio Wheat, Avalon x Cadenza
Probe	WMS350
Possible Orthologs	Xgwm350-4A [ Show all 9 ]
Probe for Locus	WMS350
Locus	Xgwm350-4A [ Show all 10 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)14 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ACCTCATCCACATGTTCTACG 3' 5' GCATGGATAGGACGCCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS350
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring