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GrainGenes Marker Report: Xgwm358.2-5D

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Locus
Xgwm358.2-5D
Type
Microsatellite
Map
Conan/Reeder-5D4.61753
[ Show Nearby Loci ]
Map Data
Wheat, Conan x Reeder
Probe
WMS358
Possible Orthologs
Xgwm358
[ Show all 7 ]



Probe for Locus
WMS358
Locus
Xgwm358.2-5D
[ Show all 8 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)18(G)2(GA)4
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' AAACAGCGGATTTCATCGAG 3'
5' TCCGCTGTTGTTCTGATCTC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS358
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring