GrainGenes Marker Report: Xgwm359-2A
Locus
Xgwm359-2A
Type
Microsatellite
Chromosome
2A
Chromosome Arm
2AS
Map
Chinese_Spring_Deletion_SSR_2A -0.89
[ Show all 7 ]
[ Show Nearby Loci ]
Breakpoint Interval
2AS5-0.78-1.00
Map Data
Wheat, Arina x Forno
[ Show all 7 ]
Species
Triticum aestivum
Probe
WMS359
Reference
Data Source
Roeder, Marion S. 98.09.15
Possible Orthologs
Xgwm359 gwm359
Probe for Locus
WMS359
Locus
Xgwm359-2A Xgwm359 YrR61 gwm359
Reference
Hao Y et al. (2011) Characterization of a major QTL for adult plant resistance to stripe rust in US soft winter wheat. Theoretical and Applied Genetics 123:1401-1411.
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. YrR61 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks
Insert contains repeat (CT)20(CTT)13imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' CTAATTGCAACAGGTCATGGG 3'
5' TACTTGTGTTCTGGGACAATGG 3' WMS359-F 5'- AGC CGC GAA ATC TAC TTT GA
WMS359-R 5'- TTA AAC GGA CAG AGC ACA CG
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C Denaturing step: 94C, 3 min. Touchdown step (decrease 1C/cycle for 15 cycles): 94C, 45 sec; 65-50C, 50 sec; 72C, 55 sec. Amplification step (30 cycles): 95C, 40 sec; 50C, 40 sec; 72C, 40 sec. Extensions step: 72C, 5 min
Polymorphism
WMS359
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm359-2A
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